<?xml version="1.0" encoding="UTF-8" ?><xml><records><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>11</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">11</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wohl, Benjamin M.</style></author><author><style face="normal" font="default" size="100%">Smith, Anton A. A.</style></author><author><style face="normal" font="default" size="100%">Kryger, Mille B. L.</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The narrow therapeutic window of ribavirin as an inhibitor of nitric oxide synthesis is broadened by macromolecular prodrugs</style></title><secondary-title><style face="normal" font="default" size="100%">Biomacromolecules</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Biomacromolecules</style></full-title></periodical><pages><style face="normal" font="default" size="100%">3916–3926</style></pages><volume><style face="normal" font="default" size="100%">14</style></volume><number><style face="normal" font="default" size="100%">11</style></number><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">8</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><isbn><style face="normal" font="default" size="100%">1525-7797</style></isbn><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1021/bm401048s</style></url></related-urls><pdf-urls><url>internal-pdf://1610159743/broadenend-therapeutic-window-PVP-small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1021/bm401048s</style></electronic-resource-num><access-date><style face="normal" font="default" size="100%">2013/10/10</style></access-date></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>8</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">8</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jensen, Bettina</style></author><author><style face="normal" font="default" size="100%">Hosta-Rigau, Leticia</style></author><author><style face="normal" font="default" size="100%">Spycher, Philipp</style></author><author><style face="normal" font="default" size="100%">Reimhult, Erik</style></author><author><style face="normal" font="default" size="100%">Stadler, Brigitte</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Lipogels: Surface-Adherent Composite Hydrogels Assembled from Poly(Vinyl Alcohol) and Liposomes</style></title><secondary-title><style face="normal" font="default" size="100%">Nanoscale</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Nanoscale</style></full-title></periodical><pages><style face="normal" font="default" size="100%">6758-6766</style></pages><volume><style face="normal" font="default" size="100%">5</style></volume><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">5</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">The Royal Society of Chemistry</style></publisher><isbn><style face="normal" font="default" size="100%">2040-3364</style></isbn><abstract><style face="normal" font="default" size="100%">Drug-eluting engineered surface coatings are of paramount importance for many biomedical applications from implantable devices to tissue engineering. Herein, we present the assembly of lipogels, composite physical hydrogels assembled from poly(vinyl alcohol) and liposomes using thiol-disulfide exchange between endgroup modified PVA and thiocholesterol containing liposomes, and the response of adhering cells to these coatings. We demonstrate the controlled loading of liposomes into the polymer matrix and the preserved mechanical properties of the lipogels. Furthermore, the lipogels are successfully rendered cell adhesive by incorporation of poly(L-lysine) into the PVA polymer matrix or by poly(dopamine) coating of the lipogels. The successful lipid uptake from the lipogels by macrophages, hepatocytes, and myoblasts was monitored by flow cytometry. Finally, the delivery of active cargo, paclitaxel, to adherent myoblasts is shown, thus illustrating the potential of the lipogels as a drug eluting interface for biomedical applications.</style></abstract><work-type><style face="normal" font="default" size="100%">10.1039/C3NR01662E</style></work-type><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1039/C3NR01662E</style></url></related-urls><pdf-urls><url>internal-pdf://0481837355/lipogels_small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1039/C3NR01662E</style></electronic-resource-num></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>4</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">4</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Andreasen, Sidsel</style></author><author><style face="normal" font="default" size="100%">Chong, Siow-Feng</style></author><author><style face="normal" font="default" size="100%">Wohl, Benjamin M.</style></author><author><style face="normal" font="default" size="100%">Goldie, Kenneth N.</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Poly(vinyl alcohol) physical hydrogel nanoparticles, not polymer solutions, exert inhibition of nitric oxide synthesis in cultured macrophages</style></title><secondary-title><style face="normal" font="default" size="100%">Biomacromolecules</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Biomacromolecules</style></full-title></periodical><pages><style face="normal" font="default" size="100%">1687–1695</style></pages><volume><style face="normal" font="default" size="100%">14</style></volume><number><style face="normal" font="default" size="100%">5</style></number><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">4</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><isbn><style face="normal" font="default" size="100%">1525-7797</style></isbn><abstract><style face="normal" font="default" size="100%">Hydrogel nanoparticles (HNP) are an emerging tool of biomedicine with unique materials characteristics, scope, and utility. These hydrated, soft colloidal carriers can penetrate through voids with dimensions narrower than the size of the particle, provide stabilization for fragile biological cargo, and allow diffusion and exchange of solutes with external phase. However, techniques to assemble HNP are few; solitary examples exist of biocompatible polymers being formulated into HNP; and knowledge on the biomedical properties of HNP remains rather cursory. In this work, we investigate assembly of HNP based on a polymer with decades of prominence in the biomedical filed, poly(vinyl alcohol), PVA. We develop a novel method for production of PVA HNP through nanoprecipitation ? based assembly of polymer nanoparticles and subsequent physical hydrogelation of the polymer. Polymer nanoparticles and HNP were visualized using scanning electron microscopy and fluorescence imaging, and characterized using dynamic light scattering and zeta potential measurements. Interaction of PVA HNP with mammalian cells was investigated using flow cytometry, viability screening, and measurements of nitric oxide production by cultured macrophages. The latter analyses revealed that PVA administered as a polymer solution or in the form of HNP resulted in no measurable increase in production of the inflammation marker. Unexpectedly, PVA HNP exerted a pronounced inhibition of NO synthesis by stimulated macrophages, i.e. had an anti-inflammatory activity. This effect was accomplished with a negligible change in the cell viability and was not observed when PVA was administered as a polymer solution. To the best of our knowledge, this is the first observation of inhibition of NO synthesis in macrophages by administered nanoparticles and specifically hydrogel nanoparticles. Taken together, our results present PVA HNP as promising colloidal hydrogel nanocarriers for biomedical applications, specifically drug delivery and assembly of intracellular biosensors.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1021/bm400369u</style></url></related-urls><pdf-urls><url>internal-pdf://0389630531/pva-nanoprecipitation-small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1021/bm400369u</style></electronic-resource-num><access-date><style face="normal" font="default" size="100%">2013/04/08</style></access-date></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>3</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">3</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Lynge, Martin E.</style></author><author><style face="normal" font="default" size="100%">Baekgaard Laursen, Marie</style></author><author><style face="normal" font="default" size="100%">Hosta-Rigau, Leticia</style></author><author><style face="normal" font="default" size="100%">Jensen, Bettina E. B.</style></author><author><style face="normal" font="default" size="100%">Ogaki, Ryosuke</style></author><author><style face="normal" font="default" size="100%">Smith, Anton A. A.</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author><author><style face="normal" font="default" size="100%">Städler, Brigitte</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Liposomes as Drug Deposits in Multilayered Polymer Films</style></title><secondary-title><style face="normal" font="default" size="100%">ACS Applied Materials &amp; Interfaces</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">ACS Applied Materials &amp; Interfaces</style></full-title></periodical><pages><style face="normal" font="default" size="100%">2967–2975</style></pages><volume><style face="normal" font="default" size="100%">5</style></volume><number><style face="normal" font="default" size="100%">8</style></number><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">3</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><isbn><style face="normal" font="default" size="100%">1944-8244</style></isbn><abstract><style face="normal" font="default" size="100%">The ex vivo growth of implantable hepatic or cardiac tissue remains a challenge and novel approaches are highly sought after. We report an approach to use liposomes embedded within multilayered films as drug deposits to deliver active cargo to adherent cells. We verify and characterize the assembly of poly(l-lysine) (PLL)/alginate, PLL/poly(l-glutamic acid), PLL/poly(methacrylic acid) (PMA), and PLL/cholesterol-modified PMA (PMAc) films, and assess the myoblast and hepatocyte adhesion to these coatings using different numbers of polyelectrolyte layers. The assembly of liposome-containing multilayered coatings is monitored by QCM-D, and the films are visualized using microscopy. The myoblast and hepatocyte adhesion to these films using PLL/PMAc or poly(styrenesulfonate) (PSS)/poly(allyl amine hydrochloride) (PAH) as capping layers is evaluated. Finally, the uptake of fluorescent lipids from the surface by these cells is demonstrated and compared. The activity of this liposome-containing coating is confirmed for both cell lines by trapping the small cytotoxic compound thiocoraline within the liposomes. It is shown that the biological response depends on the number of capping layers, and is different for the two cell lines when the compound is delivered from the surface, while it is similar when administered from solution. Taken together, we demonstrate the potential of liposomes as drug deposits in multilayered films for surface-mediated drug delivery.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1021/am4006868</style></url></related-urls><pdf-urls><url>internal-pdf://1992750416/liposome-drug-deposits-small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1021/am4006868</style></electronic-resource-num><access-date><style face="normal" font="default" size="100%">2013/04/08</style></access-date></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>2</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">2</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Andreasen, Sidsel Ø</style></author><author><style face="normal" font="default" size="100%">Chong, Siow-Feng</style></author><author><style face="normal" font="default" size="100%">Kryger, Mille B. L.</style></author><author><style face="normal" font="default" size="100%">Jensen, Bettina E. B.</style></author><author><style face="normal" font="default" size="100%">Postma, Almar</style></author><author><style face="normal" font="default" size="100%">Alves, Marie-Helene</style></author><author><style face="normal" font="default" size="100%">Städler, Brigitte</style></author><author><style face="normal" font="default" size="100%">Goldie, Kenneth N.</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Liposomal Templating, Association with Mammalian Cells, and Cytotoxicity of Poly(vinyl alcohol) Physical Hydrogel Nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">Particle &amp; Particle Systems Characterization</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Particle &amp; Particle Systems Characterization</style></full-title></periodical><pages><style face="normal" font="default" size="100%">514-522</style></pages><volume><style face="normal" font="default" size="100%">30</style></volume><number><style face="normal" font="default" size="100%">6</style></number><keywords><keyword><style face="normal" font="default" size="100%">biomaterials</style></keyword><keyword><style face="normal" font="default" size="100%">hydrogels</style></keyword><keyword><style face="normal" font="default" size="100%">liposomal templating</style></keyword><keyword><style face="normal" font="default" size="100%">nanoparticles</style></keyword><keyword><style face="normal" font="default" size="100%">poly(vinyl alcohol)</style></keyword></keywords><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">2</style></date></pub-dates></dates><isbn><style face="normal" font="default" size="100%">1521-4117</style></isbn><abstract><style face="normal" font="default" size="100%">The assembly, cellular internalization, and cytotoxicity of nanoparticles based on physical hydrogels of poly(vinyl alcohol) (PVA) are reported. PVA nanoparticles are assembled using a liposomal templating technique followed by removal of the lipids using isopropanol, a process that requires the presence of a custom-made block copolymer, poly(vinyl alcohol-b-vinyl pyrrolidone), to avoid aggregation of the nanoparticles. Polymer hydrogelation is induced via incubation in aqueous isopropyl alcohol solution, which results in PVA hydrogel nanoparticles (PVA HNP) with excellent colloidal stability and stability towards disintegration over at least 24 h. Pristine PVA HNP are found to be remarkably stealth-like and exhibit negligible cellular internalization. This feature is likely inherent with the low fouling nature of PVA and makes PVA HNP attractive for targeted drug delivery with a low level of association with non-targeted cells and tissues. Blending PVA with varied amounts of collagen results in colloidal hydrogel particles with a well pronounced tendency towards association with mammalian cells, specifically hepatocytes and endothelial cells. The association of PVA HNP elicits minimal changes in cellular proliferation, making these novel hydrogel particles convenient tools for drug delivery applications and creation of implantable artificial organelles and sensors.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1002/ppsc.201200134</style></url></related-urls><pdf-urls><url>internal-pdf://1350009240/liposomal-templating-small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1002/ppsc.201200134</style></electronic-resource-num></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>1</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">1</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kryger, Mille</style></author><author><style face="normal" font="default" size="100%">Wohl, Benjamin</style></author><author><style face="normal" font="default" size="100%">Smith, Anton</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Macromolecular Prodrugs of Ribavirin Combat Side Effects and Toxicity with No Loss of Activity of the Drug</style></title><secondary-title><style face="normal" font="default" size="100%">Chemical Communications</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Chemical Communications</style></full-title></periodical><pages><style face="normal" font="default" size="100%">2643-2645</style></pages><volume><style face="normal" font="default" size="100%">49</style></volume><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">1</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">The Royal Society of Chemistry</style></publisher><isbn><style face="normal" font="default" size="100%">1359-7345</style></isbn><abstract><style face="normal" font="default" size="100%">Chemi-enzymatic synthesis of ribavirin acrylate and subsequent RAFT co-polymerization with acrylic acid afforded a formulation of a broad spectrum antiviral drug which avoids accumulation in erythrocytes, the origin of the main side effect of ribavirin. In cultured macrophages the macromolecular prodrugs exhibited decreased toxicity while maintaining the anti-inflammatory action of ribavirin.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1039/C3CC00315A</style></url></related-urls><pdf-urls><url>internal-pdf://RBV-and-RBCs_small-1445490432/RBV-and-RBCs_small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1039/C3CC00315A</style></electronic-resource-num></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>5</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">5</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fejerskov, Betina</style></author><author><style face="normal" font="default" size="100%">Smith, Anton A. A.</style></author><author><style face="normal" font="default" size="100%">Jensen, Bettina E. B.</style></author><author><style face="normal" font="default" size="100%">Hussmann, Thomas</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioresorbable Surface Adhered Enzymatic Microreactors Based on Physical Hydrogels of Poly(vinyl alcohol)</style></title><secondary-title><style face="normal" font="default" size="100%">Langmuir</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Langmuir</style></full-title></periodical><pages><style face="normal" font="default" size="100%">344–354</style></pages><volume><style face="normal" font="default" size="100%">29</style></volume><number><style face="normal" font="default" size="100%">1</style></number><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">0</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><isbn><style face="normal" font="default" size="100%">0743-7463</style></isbn><abstract><style face="normal" font="default" size="100%">Hydrogel biomaterials based on poly(vinyl alcohol), PVA, have an extensive history of biomedical applications yet in their current form suffer from significant shortcomings, such as lack of mechanism of biodegradation and poor opportunities in controlled drug release. We investigate physical hydrogels of PVA as surface adhered materials and present biodegradable matrices equipped with innovative tools in substrate mediated drug release. Towards the final goal, PVA chains with narrow polydispersities (1.1-1.2) and molecular weights 5, 10, and 28 kDa are synthesized via controlled radical polymerization (RAFT). These molecular weights are shown to be suitably high to afford robust hydrogel matrices and at the same time suitably low to allow gradual erosion of the hydrogels with kinetics of degradation controlled via polymer macromolecular characteristics. For opportunities in controlled drug release, hydrogels are equipped with enzymatic cargo to achieve an in situ conversion of externally added prodrug into a final product thus giving rise to surface adhered enzymatic microreactors. Hydrogel-mediated enzymatic activity was investigated as a function of polymer molecular weight and concentration of solution taken for assembly of hydrogels. Taken together, we present first example of bioresorbable physical hydrogel based on PVA with engineered opportunities in substrate mediated enzymatic activity and envisioned utility in surface mediated drug delivery and tissue engineering.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1021/la3040903</style></url></related-urls><pdf-urls><url>internal-pdf://4189995068/degradable-PVA-hydrogels_small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1021/la3040903</style></electronic-resource-num><access-date><style face="normal" font="default" size="100%">2012/12/05</style></access-date></record><record><database name="Alex2013.enl" path="\\ad.nfit.au.dk\NFDFS\Users\riber\Documents\Webpage\literature\Alex2013.enl">Alex2013.enl</database><source-app name="EndNote" version="16.0">EndNote</source-app><rec-number>6</rec-number><foreign-keys><key app="EN" db-id="2av0vsdt1frt2zesasxxxdejavvs9d9xrpaw">6</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chong, Siow-Feng</style></author><author><style face="normal" font="default" size="100%">Smith, Anton A. A.</style></author><author><style face="normal" font="default" size="100%">Zelikin, Alexander N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Microstructured, Functional PVA Hydrogels through Bioconjugation with Oligopeptides under Physiological Conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Small</style></secondary-title></titles><periodical><full-title><style face="normal" font="default" size="100%">Small</style></full-title></periodical><pages><style face="normal" font="default" size="100%">942–950</style></pages><volume><style face="normal" font="default" size="100%">9</style></volume><number><style face="normal" font="default" size="100%">6</style></number><keywords><keyword><style face="normal" font="default" size="100%">microstructures</style></keyword><keyword><style face="normal" font="default" size="100%">oligopeptides</style></keyword><keyword><style face="normal" font="default" size="100%">hydrogels</style></keyword><keyword><style face="normal" font="default" size="100%">bioconjugation</style></keyword><keyword><style face="normal" font="default" size="100%">PVA</style></keyword></keywords><dates><year><style face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style face="normal" font="default" size="100%">0</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">WILEY-VCH Verlag</style></publisher><isbn><style face="normal" font="default" size="100%">1613-6829</style></isbn><abstract><style face="normal" font="default" size="100%">In this work, bioconjugation techniques are developed to achieve peptide functionalization of poly(vinyl alcohol), PVA, as both a polymer in solution and within microstructured physical hydrogels, in both cases under physiological conditions. PVA is unique in that it is one of very few polymers with excellent biocompatibility and safety and has FDA approval for clinical uses in humans. However, decades of development have documented only scant opportunities in bioconjugation with PVA. As such, materials derived thereof fail to answer the call for functional biomaterials for advanced cell culture and tissue engineering applications. To address these limitations, PVA is synthesized with terminal thiol groups and conjugated with thiolated peptides using PVA in solution. Further, microstructured, surface-adhered PVA physical hydrogels are assembled, the available conjugation sites within the hydrogels are quantified, and quantitative kinetic data are collected on peptide conjugation to the hydrogels. The success of bioconjugation in the gel phase is quantified through the use of a cell-adhesive peptide and visualization of cell adhesion on PVA hydrogels as cell culture substrates. Taken together, the presented data establish a novel paradigm in bioconjugation and functionalization of PVA physical hydrogels. Coupled with an excellent safety profile of PVA, these results deliver a superior biomaterial for diverse biomedical applications.</style></abstract><urls><related-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1002/smll.201201774</style></url></related-urls><pdf-urls><url>internal-pdf://2307342442/function-PVA-gels_small.png</url></pdf-urls></urls><electronic-resource-num><style face="normal" font="default" size="100%">10.1002/smll.201201774</style></electronic-resource-num></record></records></xml>